Image
|
Figure Caption
Figure 1
Generation of hsd11b2 mutant zebrafish line. (A) Partial (from the start codon) nucleotide and aminoacidic sequence of hsd11b2 in WT animals. (B) Partial (from the start codon) nucleotide and aminoacidic sequence of hsd11b2 in mutant animals showing in green the 19-nt insertion. (C) Representative picture of hsd11b2+/+, hsd11b2+/− and hsd11b2−/− genotyping in 2% agarose gel. (D) RT-qPCR of hsd11b2 transcripts in hsd11b2+/+ and hsd11b2−/− 5-dpf larvae. n = 4 independent biological replicas. Mean ± SEM. Statistical significance was determined by Student’s t-test. * p < 0.05.
Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Int. J. Mol. Sci.