Fig 2

(A) Confocal 20 μm z-projection of 6 dpf Tg(gfap:nucGFP);Tg(slc1a3b:myrGcAMP6-P2A-H2AmCherry) ventricle stained with GFP. GFP⁺ cells (white arrowhead) colocalize with H2AmCherry⁺ cells (white arrow). (B) Confocal 20 μm z-projection of 6 dpf Tg(gfap:nucGFP) ventricle injected with slc1a3b:nls-tdTomato. nucGFP⁺ cells (white arrowheads) colocalize with tdTomato⁺ cells (white arrows). (C) Confocal 20 μm z-projection of 6 dpf Tg(gfap:nucGFP) ventricle stained with glutamine synthetase. nucGFP⁺ cells (white arrowheads) colocalize with glutamine synthetase⁺ cells (white arrows). (D) Quantification of the percentage of gfap:nucGFP⁺ cells that express slc1a3b:nls-tdTomato (n = 124 cells) or GS (n = 76 cells). (E) Quantification of the percentage of slc1a3b:nls-tdTomato⁺ (n = 115 cells) or GS⁺ (n = 15 cells) cells that express gfap:nucGFP. (F) Confocal 10 μm z-projection of a 6-dpf Tg(slc1a3b:myrGFP-P2A-H2AmCherry)⁺ cell morphology. (G, H) Confocal 20 μm z-projections of (G) 6 dpf Tg(gfap:nucGFP) ventricle and (H) adult Tg(gfap:nucGFP) ventricle stained with Mef-2A. The nucGFP⁺ cells (white arrowhead) do not colocalize with Mef-2A puncta. Inlet area is denoted by white dotted box. (I) Quantification of percentage of gfap:nucGFP⁺/Mef-2A⁺ cells per 6 dpf (n = 211 cells) and adult ventricles (n = 49 cells). (J) Confocal 10 μm z-projection of adult Tg(gfap:gfp) ventricles stained with MBP and AcTub. MBP (white arrow) does not colocalize to gfap⁺ cells (white arrowhead). Inlet areas are denoted by white box. Data are represented as mean ± SEM. Scale bar equals 10 μm. Statistics summarized in S1 Table. See S1 Data for raw data. AcTub, acetylated tubulin; dpf, days postfertilization; GS, glutamine synthetase; MBP, myelin basic protein; V, ventricle.