ZFIN Image: Ding et al., 2021, Figure 3

Image

Figure Caption

Figure 3

TEM shows abnormal organelle morphology in smarca5-deficient RBCs.

(A) The transmission electron microscopy (TEM) view of a longitudinal section through the artery and vein plexus in sibling tail region at 2 dpf. (B) TEM view of erythrocytes in sibling embryos. The red arrow heads indicate the characteristic pattern of organization in mitochondria. (C) TEM view of a longitudinal section through the artery and vein plexus in smarca5^zko1049a tail region at 2 dpf. (D) The blue rectangular box showing blood clots in the caudal vein plexus in smarca5^zko1049a. The magnification of blood clots is shown (right). (E) TEM view of erythrocytes in smarca5^zko1049a. The red arrow heads indicate the characteristic pattern of organization in mitochondria. The disintegration of cristae in mitochondria is observed in smarca5-deficient RBCs. Ery, erythrocyte; EC, endothelial cell; Mito, mitochondria; Nuc, nucleus; Cyto, cytoplasm. (F) Scatter plots showing the section area of mitochondria relative to cell in RBCs from smarca5^zko1049a and their siblings. (G) Scatter plots showing the number of mitochondria per cell section in RBCs from smarca5^zko1049a and their siblings. Data are mean ± s.d. (F and G). Asterisk presents statistical significance (n.s. not significant). p Values were calculated by two-tailed unpaired Student’s t-test.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife