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Figure 10
Figure 10. RT-PCR analysis of atxn1 gene expression level in whole-body zebrafish. Total RNA was isolated from embryos and larvae at different developmental stages (single-cell, 3 hpf, 6 hpf, 12 hpf, 1 dpf, 2 dpf, 3 dpf, 4 dpf, 5 dpf, 7 dpf, and 10 dpf) and from adult zebrafish brains. Gene-specific primer pairs for atxn1a, atxn1b, and atxn1l were used to amplify the reverse transcribed cDNAs. The primer pair for β-actin was used as a control. The sizes of the amplicons are given.
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Full text @ Int. J. Mol. Sci.