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Figure 5
Figure 5. Atxn1l gene in zebrafish. (A) Schematic presentation of the structure of atxn1l gene on chromosome 7 minus strand. The gene consists of three exons (E1, E2, and E3) containing the coding sequence (black box) in the exons E2 and E3. (B) Only one atxn1l transcript is predicted from three exons (E1, E2, and E3; grey boxes). (C) Experimental verification of the predicted atxn1l transcript. The upper primer (U1l), specific for exon E1 sequence, in combination with the lower primer (L1l), specific for exon E3 sequence, were designed for RT-PCR of zebrafish cDNA template to amplify the 5′ end of the transcript (left panel). The length of the amplicon (right panel) and the following sequencing confirmed the predicted atxn1l transcript variant.
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Full text @ Int. J. Mol. Sci.