ZFIN Image: Matrone et al., 2021, Fig. 2

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Figure Caption

Fig. 2

Bioinformatic analysis of the S-nitrosylome in zebrafish tailfin regeneration.

A Workflow for the analysis of the S-nitrosylome. Tailfins of zebrafish (6 months old) were amputated; regrown tissue was collected in uninjured and at 3, 5, and 10 dpa (dash red lines represent the edge of the amputation); nuclear proteins were extracted and labeled with iodoTMT, digested with trypsin, and S-nitroso-peptides enriched through anti-TMT antibody containing resin and followed by LC-MS-MS. B, C Hierarchical clustering heat map and Venn diagram showing the dynamic changes in the number of S-nitrosylated nuclear proteins during the regeneration compared to uninjured. D Hallmark pathways enrichment by the differentially expressed S-nitrosylated proteins during regeneration compared to uninjured. The significant pathways are displayed along the x-axis. Dpa day post-amputation. N = 2 biological replicates, followed by bioinformatic analysis.

Acknowledgments

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