Fig. 5

Fig. 5 Jam3b promotes aggregation in vitro and an adherent morphology in vivo. (A) S2 cells transfected with constructs to express actin-GFP (control), wild-type Jam3b-mCherry or A96E mutant Jam3b-mCherry. Aggregates of cells expressing wild-type Jam3b formed within 60 ​min of rotary culture. (B) Quantification of transfected cells found within clusters relative to the total numbers of transfected cells in 3 replicate cultures. ∗∗∗, Tukey-Kramer post-hoc comparisons of means P ​< ​0.0001 (overall ANOVA, F2,6 ​= ​82.6, P ​< ​0.0001; data were arcsine-transformed for analysis to correct heterogeneity of variance among groups). (C) Membrane-targeted mCherry revealed well-spread morphologies of wild-type melanophores but more dendritic morphologies of jam3butr8e1 and igsf11^utr15e1 mutant melanophores in vivo. (D) Melanophores of jam3b^vp8re2 mutants were also dendritic but adopted a more spread appearance when expressing wild-type Jam3b transgenically. Scale bars 20 ​μM (D, for C and D).