ZFIN Image: Zhang et al., 2021, Fig. 4

Image

Figure Caption

Fig. 4

Genetic and pharmacological inhibition of AAR, but not UPR, suppresses the tars-deficiency-induced angiogenesis.

a, b Quantification and statistical analysis of the ectopic branch points per ISV of the tars^−/⁻ and sibling embryos that were treated with gcn2 MO or perk MO. c Knockout of gcn2, but not perk, suppressed the tars-deficiency-induced angiogenesis. The embryos were produced by self-cross of gcn2^−/⁻tars^+/⁻ or perk^−/⁻tars^+/− lines, and the tars^−/⁻ ones were compared with their siblings in the same litter. d Pharmacological inhibition of Gcn2 by GCN2-IN-1 and GCN2iB significantly suppressed the angiogenic phenotypes of the tars^−/⁻ embryos. e Inhibition of Perk by Perk-specific inhibitor GSK2656157 showed no significant rescue effect on the angiogenetic phenotypes. f Attenuation of UPR by 4-phenylbutyrate (4-PBA) could not rescue the angiogenetic phenotypes. In panels d–f, the solvents DMSO and H₂O were applied as negative controls, respectively. In the statistical analyses, data are presented as means ± SD; two-tailed t-test; ***P < 0.001; n.s., not significant.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Discov