ZFIN Image: Yang et al., 2021, Fig. 4

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Figure Caption

Fig. 4 Cellular adhesion inhibition restored the abnormal thyroid primordium (TP) morphology in <italic>gbp1-</italic>deficient embryos.

(a) Cartoon image show the working model of Duolink PLA strategy. (b,c) Representative images (b) and statistical assessment (c) of the β-catenin and E-cadherin interaction pattern in 4-dpf wild-type (WT) and gbp1 deficient embryos under Tg(tg:GFP) background. Means ± SEM of the three independent experiments are shown. (d–g) Representative image showing tg expression pattern (d), statistical analysis of the thyroid area (e), angle between two posterior strands (f), and length to width ratio (g) in WT and gbp1 morphants with or without LatB addition. N = 12. (h) Restoring effects on tshba expression in WT and gbp1 morphants with or without LatB addition. Means ± SEM of the three independent experiments are shown. (i,j) Representative image (i) and statistical analysis (j) showing the rescuing effects of LatB treatment on follicle number in gbp1 morphants under Tg(tg:mCherry) transgenic background. N = 12. Bars = 20 μm. (k,l) Representative image (k) and statistical analysis (l) showing the rescuing effects of LatB treatment on T4 containing units in gbp1 morphants by immunofluorescence (IF) assay. *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test).

Acknowledgments

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