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Figure 3
Figure 3. Expression of liver markers fabp10a and tfa in primary and metastatic liver tumor tissues from twist1a+/xmrk+ double transgenic zebrafish. Twist1a+/xmrk+ transgenic zebrafish were treated with 60 μg/mL Dox and 1 μg/mL 4-OHT. (A) mCherry immunofluorescence analysis of twist1a+/xmrk+ liver tumor metastasis at 4 wpi. Scale bar: 200 μm. (B) Results of semiquantitative RT-PCR showing the expression of fabp10a and tfa in primary tumor, metastatic liver tumor, and adjacent normal tissues. Actin and non-template, respectively, served as an internal control and negative control.
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