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Figure 3 App localizes to the cilia decorating the ventricle of larvae and ependymal cells in adult zebrafish. (A) Schematic representations of head and ventricle morphology in 30 hpf zebrafish larvae, dorsal view. (B) Dorsal view of ventricle immunostained for App (green) and acetylated tubulin (magenta) in 30 hpf WT zebrafish larvae. (C) Close-up of cilia (magenta) and App (green). (D) Negative controls for immunofluorescence with secondary antibodies without anti-acetylated tubulin and anti-App primary antibodies. Cell nuclei stained with DAPI (blue). (E) Schematic outline of adult zebrafish brain, lateral view. (F–G) Coronal section of adult zebrafish brain and the central canal (see asterisk). Cell nuclei labeled with DAPI (blue), acetylated tubulin (magenta), App (green). (G) Ependymal motile cilia (magenta) of the central canal with App (green) accumulation along cilia. Magnification: (B,F) = 10×, (D) = 40×, (C,G) = 60×. Scale bar: (B,D) = 50 µm, (C) = 1 µm, (F) = 500 µm, (G) = 10 µm.