Figure 5

Figure 5

met mutants exhibit reduced OPC proliferation. (A) Transverse sections of 76 hpf olig2:egfp;sox10:mrfp met^+/+ and met^uva38/uva38 larvae. Open yellow arrowheads denote sox10⁺/olig2⁺ OPCs. Dashed yellow circle denotes boundary of the spinal cord. (B–E) Quantifications of olig2⁺/sox10⁺ OLCs from ten sequential 20 μm sections of 76 hpf olig2:egfp;sox10:mrfp met^+/+ (n = 7), met^+/uva38 (n = 6), and met^uva38/uva38 (n = 6) larvae. Mean with SEM. Statistical test: one-way ANOVA with Tukey’s Multiple Comparison Test. (F) EdU treatment paradigm and lateral view spinal cord images of 74 hpf sox10:mrfp;met^+/+ and met^uva38/uva38 zebrafish larvae. Magenta-outlined yellow arrowheads denote sox10⁺/EdU⁺ OPCs. Magenta open arrowheads denote sox10⁺/EdU⁻ OLCs. Yellow open arrowheads denote sox10⁻/EdU⁺ cells. Yellow dashed line denotes ventral edge of the spinal cord. (G) Quantifications of sox10⁺/EdU⁺ OLCs from spinal cord images of 76 hpf sox10:mrfp EdU labeled met^+/+ (n = 7), met^+/uva38 (n = 8), and met^uva38/uva38 (n = 7) larvae. Mean with SEM. Statistical test: one-way ANOVA with Tukey’s Multiple Comparison Test. Scale bars (A) 10 μm, (F) 20 μm.