Figure 3

Figure 3

The deficiency of FARS2 impairs cell motility, proliferation, invasion, and tube formation in human umbilical vein endothelial cells (HUVECs). (A) Representative images of scratch-wound assays of HUVECs 0 and 6 h at 48 h after transfection with a control (siCtrl) or FARS2-specific (si-FARS2) siRNA. Scale bar = 200 μm. (B) Quantification of the healed wound area from (A). Data are prepresented as the mean and SEM (n = 10). ^***P < 0.001 via ANOVA. (C) A CCK8-based cell proliferation assay of HUVECs at the indicated time-points after transfection with siCtrl or si-FARS2. The measurements were made in triplicate (mean and SEM), and the results are indicative of three independent experiments. ^****P < 0.0001. (D) Representative images of transwell-based migration assays of HUVECs 48 h after transfection with siCtrl or si-FARS2. Scale bar = 200 μm. (E) Quantification of the number of migrated cells from (D). ^***P < 0.001. (F) Representative images of tube network assays of HUVECs 48 h after transfection with siCtrl or si-FARS2. Scale bar = 200 μm. (G,H) Quantification of the branching points (G) and tube lengths (H) from (F). The measurements were made in triplicate (mean and SEM), and the results are indicative of three independent experiments. ^****P < 0.0001.