Figure 1

Figure 1

Expression pattern and specificity of Ca8:eGFP in the Purkinje cells of valvula cerebelli. (a) Sagittal and coronal sections show the specificity of the eGFP expression in all Purkinje cells (green) of the adult zebrafish cerebellum. (b) All eGFP-expressing Purkinje cells (green) are ZebrinII, PV, and GABA positive (magenta). Arrowheads indicate double-labeled cells. (c) Regionalized quantification of the Purkinje cell soma size in the adult zebrafish cerebellum. CCe corpus cerebelli, CDF cumulative distribution frequencies, GABA γ-aminobutyric acid, LCa lobus caudalis cerebelli, GFP green fluorescent protein, PC Purkinje cell, PV parvalbumin, Va valvular cerebelli, Val lateral part of valvular cerebelli, Vam medial part of valvular cerebelli. Data are presented as violin plots showing the median with quartiles. ****P < 0.0001; ns, not significant. For detailed statistics, see Supplementary Table S1.