ZFIN Image: Shimizu et al., 2021, Figure 3

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Figure 3

Overexpression of VDAC1 or VDAC2 but not VDAC3 restores rhythmic cardiac contractions in tremblor/ncx1h mutants. (A) Western blot with anti-FLAG antibody after SDS-PAGE with lysate from 32 hpf uninjected embryos or embryos injected with 25 pg. of C-terminally FLAG-tagged VDAC mRNA demonstrates that all VDAC mRNAs are expressed at comparable levels in embryos. β-actin was used as a loading control. (B) While injection of 25 pg. VDAC1 or VDAC2 mRNA significantly restored rhythmic cardiac contractions in 1-day-old tremblor/ncx1h mutant embryos (46.5 ± 3.4%, N = 3, n = 120, and 44.8 ± 0.4%, N = 3, n = 134, respectively, as opposed to 14 ± 3.5%, N = 3, n = 175 in uninjected siblings), injection of 25 pg. VDAC3 failed to recapitulate this effect (19.4 ± 1.1%, N = 3, n = 114). Overall rescue percentages represent the mean rescue percentage ± s.e.m. from N independent experiments, using a total of n embryos.

Acknowledgments

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