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Figure 5
Expression of krasV12 with LPS treatment enhanced the increase in p-AKT in intestinal epithelial cells. (A,C) Immunofluorescence staining (red) was performed on intestinal paraffin sections of WT (N = 20), WT/LPS (N = 20), kras+ (N = 20), and kras+/LPS (N = 20) zebrafish. (B,D) Immunofluorescence staining of p-AKT and p-ERK as a marker of RAS signaling and quantification of the number of positive cells. Differences among the variables were assessed using Student’s t-tests. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar: 50 μm.
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