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Figure 4
Treatment of homozygous sacs?/? mutants with TUDCA and ADLL is able to rescue the locomotor impairment seen in sacs?/? larvae. (A,B) Automated analysis of spontaneous motor activity of sacs?/? after drug treatments (untreated sacs?/? n = 100; sacs?/? mutants treated with ADLL/Tanganil? n = 150; sacs?/? mutants treated with TUDCA n = 150; controls n = 167) in 4 independent experiments. * p ? 0.05, ** p ? 0.01, *** p ? 0.001, **** p ? 0.0001were calculated by the Mann-Whitney test. (C) Swimming pattern of sacs?/? after drug treatments (untreated sacs?/? n = 34; sacs?/? mutants treated with ADLL/Tanganil? n = 34; sacs?/? mutants treated with TUDCA n = 34; controls n = 34). Each point in the graph represents the mean ± standard error of the mean of the distance moved by zebrafish larvae in 2-min time bins. The shaded parts represent the dark and the unshaded parts the light periods. The total number of embryos used for each group tested was 36. (D) Between-group differences in the average total activity were evaluated by comparing 1 min after and 1 min before light-to-dark and dark-to-light transitions. Values are represented as means ± standard error of the means. (E,E?) The average of the total activity of each group was measured during light on (E) and light off (E?) conditions. * p ? 0.05, calculated by Dunnett?s multiple comparisons test. Abbreviations: n, number of evaluated embryos in total; ns, not significant.