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Figure 6
Impact of LCACs on cardiomyocyte numbers in zebrafish embryos. (A–H) Red-fluorescence-positive cardiomyocytes (CMs) of zebrafish embryos in wild-type and 0.1 M or 0.5 µM carnitine-treated embryos. (I–K) Counting CMs of whole heart, ventricle and atrium from wt and C3-, C18- and C18:1-treated embryos (total CMs: wt: 319.33 ± 26.08, C3 0.1 µM: 304.67 ± 7.57, C18 0.1 µM: 311.67 ± 5.51, C18:1 0.1 µM: 310.67 ± 17.56, C3 0.5 µM: 320.67 ± 21.78, C18 0.5 µM: 333.33 ± 37.17, C18:1 0.5 µM: 279.0 ± 55.75, SD, n = 3, ns: p > 0.05; ventricular CMs: wt: 215.67 ± 20.74, C3 0.1 µM: 211.67 ± 11.37, C18 0.1 µM: 215.67 ± 8.33, C18:1 0.1 µM: 220.67 ± 12.34, C3 0.5 µM: 227.67 ± 15.01, C18 0.5 µM: 232.67 ± 16.62, C18:1 0.5 µM: 214.67 ± 8.15, SD, n = 3, ns: p > 0.05; atrial CMs: wt: 103.67 ± 7.02, C3 0.1 µM: 93.0 ± 4.0, C18 0.1 µM: 96.0 ± 13.75, C18:1 0.1 µM: 90.0 ± 17.44, C3 0.5 µM: 93.0 ± 14.73, C18 0.5 µM: 99.67 ± 19.73, C18:1 0.5 µM: 97.67 ± 10.41, SD, n = 3, ns: p > 0.05). Abbreviations: ns = not significant, wt =wild-type.