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Figure 2
RDH12 protects cells from atRAL-induced oxidative stress. (A) Dosing untransfected cells with 50 µM atRAL for 24 h causes a significant reduction in SOD activity, indicating an increase in oxidative stress. Cell expressing WT RDH12 are protected from atRAL-induced oxidative stress. Dosing with atRAL causes a significant reduction in SOD activity in mutant cells. Statistical significance was analysed using two-way ANOVA and Sidak’s multiple comparison test. ** p ≤ 0.01, *** p ≤ 0.001. (B) Expression of oxidative stress markers NRF2, SOD2, CAT, GPX1, and HO-1 was analysed by RT-qPCR following treatment with 50 µM atRAL for 24 h. Statistical significance was analysed with paired t-test. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.