ZFIN Image: Luo et al., 2021, Fig. 3

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Fig. 3

Pten mutagenesis in liver tissue disrupts liver morphology and initiates hepatocarcinogenesis. a Abundances of Ptena, Ptenb, and Fabp10 mRNA in liver tissues of WT, fabp10^WT, and pten KO fish (n = 3 per group). b Western blot analyses of Pten and Fabp10 expression in the liver tissues of WT, fabp10^WT, and pten KO fish (n = 3 per group). c Overall survival rates of WT, fabp10^WT, and pten KO fish (n = 200 per group). d Gross morphology of 3-month-old fabp10^WT (di) and pten KO fish (dvi). Representative bright field images of the internal abdominal organs, with the liver outlined, in 3-month-old fabp10^WT (dii) and pten KO fish (dvii). in, intestine; li, liver tissues, sb, swim bladder. Histological examination of the liver tissues from fabp10^WT (diii-dv) and pten KO fish (dviii-dx) at 3 and 6 months of age, respectively. Several typical hepatocarcinogenesis phenotypes were observed in 3- and 6-month-old pten KO fish, including abnormal lipid accumulation in hepatocytes (broken black boxes; Fig. 3diii, 3dviii), hepatocytes abnormal (black arrows; div, ix), disordered hepatic plates (blue arrows; dv, x), and veins disappearance (green arrowheads; dv, x). Scale bars, 100 μm. e Immunohistochemical staining was performed to examine the expression of several key tumour-related factors, including Gs, Pcna, and pAkt, in liver tissues from 3-month-old fabp10^WT and pten KO fish. Scale bars, 100 μm. The data are shown as the mean ± SEM values. *p < 0.05, **p < 0.01

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Exp. Clin. Cancer Res.