Fig. 5

Fig. 5

a qPCR data examining cx41.8 expression (fold change relative to expression in kdrl:GFP⁺ head subset) in FACS-sorted cells from kdrl:GFP, ikaros:GFP and mpeg1:GFP embryos. GFP⁺ cells from dissected heads and tails were sorted from 48 hpf embryos. b For each transgenic line have been pooled n = 20 animals, and each experiment was repeated independently three times. Statistical analysis was completed using a one-way ANOVA, with Tukey–Kramer post hoc tests, adjusted for multiple comparisons *P = 0.018; **P = 0.001; (n.s.) non-significant P = 0.26; P = 0.74. ccmyb expression at 60 hpf in wild type and cx41.8^−/− embryos, and after GSH treatment. d Quantification of cmyb-expressing cells. Statistical analysis: one-way ANOVA, with Tukey–Kramer post hoc tests, adjusted for multiple comparisons, *P = 0.045; ****P < .0001. Center values denote the mean, and error values denote s.e.m. e Imaged area in the tail at 48 hpf, as indicated by the black dotted line. Confocal imaging of the CHT at 48 hpf of cmyb:GFP⁺ cells (green), CellROX fluorescent probe (red) and merge, in NT and after heptanol treatment cmyb:GFP embryos. Orthogonal projection CellROX⁺cmyb:GFP⁺ double-positive cell after heptanol treatment. Scale bars: 100 μm (c); 25 μm (e).