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Figure 5 Figure 5 Validation of differential expression by qRT-PCR. (A) The expressions of candidate DEGs for histone variants confirmed by qRT-PCR. (B) The expressions of candidate DEGs for bile acid metabolism-related genes confirmed by qRT-PCR. Data represent the means ± the SEM and are tested for statistical significance using two-tailed student’s t-test. *, p < 0.05; **, p < 0.01; ns, not significant.
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