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Figure 4—figure supplement 1. (A–D) Lateral confocal projections of Tg(6xHsa.NFKB:EGFP)nc1 embryos reporting NfkB signalling levels of hai1afr26 at 24hpf (B), hai1ahi2217 at 15hpf, and siblings at 24hpf (A) and 15hpf (C). The increase in NfkB reporter signal occurs after overt phenotype onset. (E) Nature of the ikbkgsq304 mutant allele showing TALEN site location within the intron-exon structure of the gene (coding and non-coding exons depicted as filled and open boxes, respectively). Sequence of part of exon 3 shown below with the TALEN binding site in red. The 14 bp deletion in the ikbkgsq304 allele is indicated under the WT sequence as dashes. This leads to a frameshift changing codon 80 from GGC (Gly) to GGT (Val), then nine aberrant amino acids followed by a stop codon. (F, G) Lateral widefield fluorescent images of hai1ahi2217; ikbkg+/sq304 (F) and hai1ahi2217; ikbkgsq304 (G) embryos at 24hpf stained with pentafluorobenzenesulfonyl fluorescein (PFBSF) showing no loss of H2O2 in hai1a mutants upon mutation of ikbkg. (H, I) Lateral confocal projections of 48hpf hai1afr26; Tg(6xHsa.NFKB:EGFP)nc1 embryos, treated with DMSO (H) or with 2.5 µM 2-APB (I). Scale bars: (A, H) = 200 µm; (C) = 100 µm; (F) = 50 µm.