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Figure 2—figure supplement 1. (A, B) Sequence chromatograms of part of exon 6 of the zebrafish st14a gene from WT (A) and the st14asq10 allele showing the 5 bp insertion highlighted by red bar. (C) Effect of the 5 bp insertion (red) in the sq10 allele leads to alteration of reading frame, eight aberrant amino acids followed by a termination codon (red lettering). The codon altered to nonsense codon is underlined in both WT and mutant sequences. (D) Box and whiskers plot of pentafluorobenzenesulfonyl fluorescein (PFBSF) fluorescent staining intensity of 24hpf WT, hai1ahi2217 mutants, hai1ah12217; st14asq10 double mutants, and hai1ahi2217 injected with duox MO. n = 8; ANOVA with Bonferroni post-test ***p<0.001, **p<0.01. (E) TP63 (magenta) and eGFP (green) antibody staining (left column) with DIC imaging (right column) for 48hpf hai1ahi2217; Tg(mpx:eGFP)i114 mutants either untreated (top row) or treated with 40 µM diphenyleneiodonium (DPI) (bottom row). Scale bar: (D) = 100 µm.