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Figure 2 (A) Schematic of the Matriptase1a protein with domains given and transmembrane domain as grey line. Location and nature of the sq10 allele given by red arrow. (B, C) Lateral DIC (Differential Interference Contrast) images of st14a+/sq10 (B) and MZ st14asq10 (C) otic vesicles at 24hpf showing absence of otoliths (arrowheads in B) in the maternal zygotic st14a mutants. (D, E) Lateral DIC images of hai1ahi2217 single mutant (D) and st14asq10; hai1ah12217 double mutant (E) at 48hpf highlighting rescue of epidermal aggregates and fin morphology (arrowheads) in the double mutants. (F) Projected confocal images of pentafluorobenzenesulfonyl fluorescein (PFBSF) staining at 24hpf (left column), TP63 (magenta), and eGFP (green) antibody staining at 48hpf (middle column) with DIC imaging (right column) for hai1ahi2217 single mutants (top row), st14asq10; hai1ahi2217 double mutants (middle row), and hai1ahi2217 mutants injected with 0.4 mM, duox MO + 0.2 mM tp53 morpholino (bottom row). Individuals for middle and right columns were hemizygous for the Tg(mpx:eGFP)i114 transgene. (G) Counts of eGFP-positive neutrophils on the fins of hai1ahi2217; Tg(mpx:eGFP)i114 or Tg(mpx:eGFP)i114, and either uninjected, injected with morpholino against duox (left side of graph), treated with 0.5% DMSO (Dimethyl sulfoxide) or 40 µM diphenyleneiodonium (DPI) (right side of graph). n = 10; t-test; ***p<0.001; *p<0.05. Scale bars: (C) = 20 µm; (E, F) = 100 µm.