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Figure 1—figure supplement 1. (A, B) Tracks of neutrophil migration taken from Video 2 in WT (A) and hai1ahi2217 (B) from immediately after wounding tail fin at indicated site. (C, D) Graph of neutrophil speed (C) and distance travelled (D) before and after wounding in WT and hai1ahi2217. Neutrophils in wounded WT move as fast as unwounded hai1a mutants. Wounding hai1a mutants accelerates neutrophils. n = 15; ANOVA with Bonferroni post-test ***p<0.001, **p<0.01. (E, F) Projected confocal images showing neutrophils (green, labelled by Tg(fli1:egfp)y1 transgenic line) are not attracted to regions of apoptosis (arrowhead, red, stained by TUNEL), but to epidermal aggregates (arrow, magenta, stained by anti-TP63) in 24hpf hai1ahi2217 mutants (F) but not WT (E). (G, H) Sequence chromatograms of genomic DNA from WT (upper panels) and dandruff alleles ti251 (G) and t419 (H) (lower panels). Altered codon is underlined in red with altered base indicated by arrow. (I) ClustalW protein alignment of the amino acid substitution in the ti251 allele showing broad conservation of across vertebrates. (J, K) Selected regions of 2D gel of protein extracted from 48hpf embryos for hai1at419 (J) or hai1ati251 (K) in red, superimposed over WT protein samples (green in both). The shift in pI of Peroxiredoxin4 in both alleles is indicated with an arrow. (L) Box and whiskers plot of pentafluorobenzenesulfonyl fluorescein (PFBSF) fluorescent staining intensity of WT and hai1afr26 mutants at 24hpf in trunk and tail. n = 9; t-test ***p<0.001. (M) Lateral projected confocal views of PFBSF staining of WT (upper row) and hai1afr26 (lower row) trunks and tails at 24hpf and 48hpf. Scale bars (F) = 100 µm; (M) = 50 µm.