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Figure 1 (A) A plot overview showing the entire light-dark transition test applied for the CBZ experiment, after 0–120-hpf exposure. The blue horizontal bar indicates the acclimatization period, while the grey and black bars represent the light and dark period, respectively. The X-axis shows the whole experiment time, whereas the Y-axis shows the time (s) spent in activity within 1 min by the zebrafish embryos (n = 12). Each dot shows the average time (s) spent in activity by 12 embryos/concentration in 1 min of recording, whereas different colours represent different concentrations shown in the upper left legend. (B) A dose–response curve of an individual CBZ experiment. The data set belongs to the first light-dark block of Figure 1A. The red crosses/lines represent the light period, whereas the black triangles/lines represent the dark period. The X-axis shows the CBZ dose range (µM) in log scale, whereas the Y-axis shows the time (s) spent in activity within 1 min. Each small symbol shows the time (s) spent in activity by each of the individual 12 replicate embryos within 1 min, while the large symbol represents the geometric means of n = 12 together with their confidence intervals (error bars).