ZFIN Image: Hsiao et al., 2021, FIGURE 3

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Figure Caption

FIGURE 3

Developmental timing-specific analysis for the cell proliferation and cell cycle in developing larvae. The larvae of control and FD groups at 26, 48, and 72 hpf were subjected to immunostaining with anti-PH3 antibodies (M-phase marker) (A), as well as TUNEL assay for apoptosis (B). The number of PH3+ cells and apoptotic cells within eye area were quantified by counting the cells with fluorescent signal using the on-line software Image J (C). Larvae at 26 hpf (D) and 72 hpf (E) were dispersed, stained with propidium iodide (PI) and analyzed for cell cycle with flow-cytometry and quantified. White arrows indicate the signals of PH3-positive cells; yellow arrows represent the signals of apoptosis; red dotted lines circle the eye area. Statistical data are shown in mean ± SEM. **p-value < 0.01; *p-value < 0.05.

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Acknowledgments

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