ZFIN Image: Mikdache et al., 2021, Figure 1

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Figure Caption

Figure 1

Characterization of rgs4 mutant. (A) Schematic representation of the rgs4 genomic locus. The extended region on the exon 2 represents the sequence targeted by the CRISPR/Cas9 system. Red: sgRNA binding site. Blue: PAM sequence. Rgs4⁺ corresponds to the wild-type allele; rgs4⁻ is the loss-of-function allele used in this study. Dashes represent the 2 base pairs deletion. (B) Schematic of the wild type Rgs4 protein (Rgs4⁺) and the mutated Rgs4 protein (Rgs4⁻). The 215 amino acid (aa) long Rgs4⁺ protein contains the RGS domain from amino acid 71 to 183. In rgs4^−/− mutant fish, the 2 base pairs deletion results in a frame shift from the amino acid 28 generating a premature STOP codon at the level of the amino acid 64. (C) Lateral views of WT and MZrgs4 embryos at 48 hpf; black arrow designates a slight heart oedema observed in MZrgs4 embryos. Scale bar = 200 µm.

Figure Data

Phenotype details

Acknowledgments

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