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Fig. 2 Expression of stm, survival rate and abnormalities of otoliths in stm−/− mutant. (A) Relative expression of stm in embryos of WT and stm−/− mutant were compared. mRNA abundance was measured in triplicate for each sample from three different paring, and all data were normalized by the number of elongation factor 1α (EF1α) transcripts in each sample. (B) Survival rates of embryos in the WT and stm−/− mutant (F6 generation) at 5 dpf. n = total number of embryos; N = number of biological replicates (embryos from different pair of fishes). (C) Percentages of abnormal otolith containing embryos in the WT and stm−/− mutant. n = total number of embryos; N = number of biological replicates (embryos from different pair of fishes). Representative otoliths of 5 dpf embryos in WT and stm−/− mutant are indicated. Scale bars are 100 µm. Asterisks represent significant difference between the samples (**P ≤ 0.001).