Fig. 1.

Fig. 1.

ccdc103 is expressed in myeloid cells. (A,B) ccdc103 expression in a 17-somite stage (ss) wild-type (WT) embryo. hp, hematovascular progenitors (black arrowheads); pn, pronephros (yellow arrowhead). (C,D) The anterior domain of ccdc103 expression (black arrowheads) is expanded in embryos co-injected with scl and lmo2 mRNAs. In A, C and D, images are lateral views with anterior left. In B, image is a dorsal view. (E) RT-PCR for ccdc103, l-plastin (positive control) and gapdh performed on cDNA isolated from fluorescence-activated cell sorting (FACS)-sorted spi1b:EGFP⁺ zebrafish myeloid progenitor cells. (F) RT-qPCR for ccdc103 and gata1 performed on cDNA isolated from embryos injected with spi1b mRNA. *P<0.05 (two-tailed unpaired Student's t-test). (G) RT-PCR for human CCDC103 was performed with cDNA isolated from HL-60 cells, CD34⁺/CD38^– hematopoietic stem cells (HSCs) and cord blood. (H-H″) Immunohistochemistry (IHC) for Ccdc103 (arrows), GFP and DAPI in an mpx:GFP⁺ zebrafish neutrophil at 24 hpf. Scale bars: 100 μm (A-D), 10 μm (H).