ZFIN Image: Karampelias et al., 2021, Fig. 7

Image

Figure Caption

Fig. 7 Folinic acid stimulates β-cell differentiation in neonatal pig islets.

a–d Folinic acid stimulates β-cell differentiation from ductal cells in neonatal pig islets. Images of control (a) and folinic acid-treated (b) neonatal pig islets immunostained for insulin and CK7. Quantification of the percentage of CK7⁺ and CK7⁺Insulin⁺ cells is shown in (c) and (d), respectively. Arrowheads point to CK7⁺Insulin⁺ cells. n = 4 biologically independent pig islet preparations. Data are presented as mean values ± SEM. d *P = 0.0286. e–h Folinic acid did not increase the percentage of α- or β-cells in the in vitro neonatal pig islet culture. Control (e) and folinic acid-treated (f) neonatal pig islets immunostained for insulin and glucagon. Quantification of the percentage of α- and β-cells is shown in (g) and (h), respectively. n = 4 biologically independent pig islet preparations. Data are presented as mean values ± SEM. The scale bar is 50 μm for all pictures in the figure. All statistical analyses were performed with a Mann–Whitney two-tailed test.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.