Fig. 7

Fig. 7

a Example of low-speed movement in a WT larva (green), high-speed movement in the same WT larva (orange), and seizure-like movement in a PTZ-treated larva (red). Top traces represent the larvae track during 15 min recording in a 96-well plate. The bottom panels show speed values across time for the events highlighted. Note the short and long duration in the high-speed events in WT and PTZ-treated larvae, respectively. b Distribution of maximum speed and duration across all movements in WT (N = 109 larvae) during the 15-minute recording session. The average maximum speed was 10.5 mm/sec and the duration of the events was <1 s. c Frequency of seizure-like movements (defined as events with maximum speed >28 mm/sec and duration >1 s) in control and PTZ-treated larvae at different concentrations after 10, 30, and 60 minutes (two-way ANOVA p < 0.05). Note the increased number of events with increasing PTZ dose and the lower number when using 15 mM after 60 minutes owing to increased larvae mortality. Data displayed as mean ± SEM.