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Fig. 1 Overexpression of wild-type but not enzyme dead CK2? promotes the onset of MYC-induced acute lymphoblastic leukemia in zebrafish. (A) Diagram of the experimental design. (B) Thymic fluorescence in the Tg(rag2:MYC-ER;lck:EGFP) (left), Tg(rag2:MYC-ER;lck:EGFP;lck:CK2?wt; rag2:mCherry) (middle), and Tg(rag2:MYC-ER;lck:EGFP;lck:CK2?K68M;rag2:mCherry) (right) fish raised in 129 nM 4-hydroxytamoxifen (4HT) at 12 weeks of life. One representative fish is shown for each group. (C) Kaplan-Meier analysis of tumor-free fish revealed that overexpression of CK2?wt but not CK2?K68M significantly accelerated the onset of MYC-induced acute lymphoblastic leukemia (ALL) (P=0.0013 for Tg(rag2:MYC-ER;lck:EGFP) [MYC-ER; green line] vs. Tg(rag2:MYCER; lck:EGFP;lck:CK2?wt; rag2:mCherry); [MYC-ER;CK2?wt; red line] n=19 and n=22, respectively; and P=0.0008 for MYC-ER;CK2?wt [red line] vs.Tg(rag2:MYCER; lck:EGFP;lck:CK2?K68M;rag2:mCherry) [MYC-ER;CK2?K68M; black line], n=22 and n=13, respectively). There was no statistical significance between MYCER and MYC-ER;CK2?K68M fish. Statistical analysis was performed using the log-rank test. The scale bar in the left and middle panel of Figure 1B =1 mm and in the right panel =200 mm.