IMAGE

Fig. 1

ID
ZDB-IMAGE-210603-18
Source
Figures for Zhou et al., 2020
Image
Figure Caption

Fig. 1 Overexpression of wild-type but not enzyme dead CK2α promotes the onset of MYC-induced acute lymphoblastic leukemia in zebrafish. (A) Diagram of the experimental design. (B) Thymic fluorescence in the Tg(rag2:MYC-ER;lck:EGFP) (left), Tg(rag2:MYC-ER;lck:EGFP;lck:CK2αwt; rag2:mCherry) (middle), and Tg(rag2:MYC-ER;lck:EGFP;lck:CK2αK68M;rag2:mCherry) (right) fish raised in 129 nM 4-hydroxytamoxifen (4HT) at 12 weeks of life. One representative fish is shown for each group. (C) Kaplan-Meier analysis of tumor-free fish revealed that overexpression of CK2αwt but not CK2αK68M significantly accelerated the onset of MYC-induced acute lymphoblastic leukemia (ALL) (P=0.0013 for Tg(rag2:MYC-ER;lck:EGFP) [MYC-ER; green line] vs. Tg(rag2:MYCER; lck:EGFP;lck:CK2αwt; rag2:mCherry); [MYC-ER;CK2αwt; red line] n=19 and n=22, respectively; and P=0.0008 for MYC-ER;CK2αwt [red line] vs.Tg(rag2:MYCER; lck:EGFP;lck:CK2αK68M;rag2:mCherry) [MYC-ER;CK2αK68M; black line], n=22 and n=13, respectively). There was no statistical significance between MYCER and MYC-ER;CK2αK68M fish. Statistical analysis was performed using the log-rank test. The scale bar in the left and middle panel of Figure 1B =1 mm and in the right panel =200 mm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Haematologica