Figure 2

Figure 2

WT and mutant TTLL11 mRNA expression after starvation. WT (DE0194) and mutant (DE0193) fibroblasts were treated with medium without FBS for different times. Cells were then processed for mRNA isolation and the relative mRNA expression of both transcript 1 (A) and 2 (B) of TTLL11 at different time point after serum starvation were determined by qPCR. The levels of mRNAs were plotted relative to cells harvested at 0 h (n = 3). Error bars represent SD. The difference between one independent group and the control group 0 h was examined by unpaired, two-tailed Student’s t-test, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.