ZFIN Image: Sofou et al., 2021, Figure EV4

Image

Figure Caption

Figure EV4 Analysis of endolysosomal compartments (related to Figs 4 and 5)

A

Quantification of cellular uptake of fluorescently labeled transferrin in fibroblast lysates, normalized to total protein levels (n = 3 biological replicates). Data represented as mean ±SEM. Statistical comparisons by unpaired two‐tailed Student’s t‐test (n = 3; P = 0.0023). **P < 0.01.

B

Immunoblot (left) of RAB11B in the indicated fibroblasts, under basal and serum‐starved conditions, and (right) summary quantifications normalized to levels of actin (ACTB) and expressed as % of controls. Bar graphs represent data as mean ±SEM (n = 3 biological replicates).

C

Representative confocal micrographs of fibroblasts stained for LAMP2 and LysoTracker. Scale bar 10 μm.

D

Quantification of the co‐localization between LysoTracker and LAMP2, expressed as Pearson correlation coefficients. Colored horizontal bars indicate the median values and whiskers 5 and 95 percentiles (n = 10 optical sections from three independent experiments).

E

Quantification of the number and intensities of LAMP2‐stained puncta. Data represented as mean ± SEM (n = 6).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.