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Figure 3 Hearts of adult tnnt2a+/RK94del display increased myocardial stress and fibrosis. (A–C) In situ hybridization on 4-month-old adult heart sections of tnnt2a+/+, tnnt2a+/RK94del, and tnnt2a+/hu11260 stained for nppb, a marker of myocardial stress. In situ hybridization on adult heart sections of tnnt2a+/+ and tnnt2a+/RK94del stained for (D,E) tnnt2a, to mark cardiac Troponin T expression, and (F,G) col6a1, which marks collagen. (H,I) AFOG staining, which marks collagen in blue, muscle in orange, and fibrin in red of tnnt2a+/+ and tnnt2a+/RK94del. (J,K) In situ hybridization on adult heart sections of tnnt2a+/+ and tnnt2a+/RK94del stained for postnb, to mark fibroblasts. Images were taken at a magnification of 20×, except for (H,I) where magnification was at 60×. Scale bars are 200 µm for whole-heart tile scans, 50 µm for zoom-in regions, and 20 µm for zoom-in regions of H, I. A: atrium, V: ventricle, BA: bulbus arteriosus.