ZFIN Image: Lu et al., 2021, Fig. 4

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Figure Caption

Fig. 4 Effect of CA on cell autophagy. (A) Effects of DOX (1 and 10 μM) and CA (5, 50, 100, and 300 μM) alone on viability of H9C2 cells at 24 h by MTT assay (n = 3). (B) Atg7 was confirmed knockdown via RT-PCR in HeLa cells. (C) Quantitative analysis of yellow puncta (autophagosomes) and red puncta (autolysosomes). Data are expressed as mean ± SD. *P < 0.05 (D) Wild-type (Control) and atg7-knockout (SiRNA-atg7) HeLa cells were transiently transfected with pBABE-puro mCherry-EGFP-LC3B plasmid and then untreated or treated with CA (50 μM) for 24 h. Representative images showing EGFP-LC3 and mCherry-LC3 puncta. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Acknowledgments

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