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Fig. 2 phoenixin/smim20 is expressed in the brain of adult zebrafish. (A) Diagram of sagittal section of generalized adult fish brain, anterior to the left, the location of GnRH cells (red; in fishes with GnRH cells). (B–J) In situ hybridization using phoenixin/smim20 probe, 20 μm sagittal cryosections, anterior to the left. Insets show higher magnification of cells expressing phoenixin/smim20. phoenixin/smim20 is expressed distinct brain regions outside the hypothalamic-pituitary axis including (B) olfactory bulb (OB), (C) telencephalon (Tel), (D) tectum opticum (TeO), and (E) the corpus cerebelli (CCe) of the cerebellum. Within the preoptic area (F) expression was observed in the magnocellular pre-optic nucleus (PM), posterior parvocellular pre-optic nucleus (PPp), suprachiasmatic nucleus (SC) and chiasma opticum (CO). Strong hypothalamic expression of phoenixin/smim20 was observed in (G) the ventral zone of the periventricular hypothalamus (Hv), and (H) the caudal zone of periventricular hypothalamus (Hc) and dorsal zones of periventricular hypothalamus, (DIL). Expression was also observed in the pituitary (I). phoenixin/smim20 sense probe (J) shows no labeling. Scale bars: (B–I) 100 μm; (J) = 50 μm; boxed areas = 20 μm. High magnification views are shown in boxed areas. (A modified from Whitlock et al., 2019).
Reprinted from Gene expression patterns : GEP, 39, Ceriani, R., Calfún, C., Whitlock, K.E., phoenixin(smim20), a gene coding for a novel reproductive ligand, is expressed in the brain of adult zebrafish, 119164, Copyright (2020) with permission from Elsevier. Full text @ Gene Expr. Patterns