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Fig. 1 Characterization of zebrafish phoenixin/smim20. (A) Amino acid sequence alignment of Phoenixin in spotted scat, (Scatophagus argus), spotted gar, (Lepisosteus oculatus), humans (Homo sapiens), mouse (Mus musculus), chicken, (Gallus gallus) common lizard, (Zootoca vivipara), western clawed frog, (Xenopus tropicalis), Coelacanth, (Latimeria chalumnae) and elephant shark, (Callorhinchus milii). Homo sapiens, Mus musculus, Gallus gallus, Zootoca vivipara and Scatophagus argus contain amidation sites (rectangles). Within the PNX amino acid sequence cleavage sites are indicated (arrowheads) and predicted (black) and confirmed (red) peptides of varying lengths are indicated (top) (Yosten et al., 2013). (B) phoenixin/smim20 is expressed in embryos and juvenile zebrafish. RT-PCR from cDNA samples of 3 dpf, 7dpf, 1mpf and of the pre-optic area (POA) of adult fish. +, -: genomic DNA from POA of adult zebrafish. Housekeeping gene: beta actin.
Reprinted from Gene expression patterns : GEP, 39, Ceriani, R., Calfún, C., Whitlock, K.E., phoenixin(smim20), a gene coding for a novel reproductive ligand, is expressed in the brain of adult zebrafish, 119164, Copyright (2020) with permission from Elsevier. Full text @ Gene Expr. Patterns