ZFIN Image: Inoue et al., 2021, Figure 2

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Figure Caption

Figure 2

Larsb-knockout larvae display severe developmental phenotype and liver abnormality with early lethality. (A) Bright field lateral views of larsb^+/+ and larsb^−/− embryos at 96 h post fertilization (hpf). Scale bar: 500 μm (top row) and 300 μm (bottom row). (B) Kaplan–Meier survival curve of larsb^+/+ (n = 32), larsb^+/- (n = 63), and larsb^−/− (n = 23) larvae. (C) Lateral views of larsb^+/+ and larsb^−/− embryos containing haemoglobin-containing cells (white arrows) stained with o-dianisidine at 72 hpf. (D) Morphological abnormality at 3 dpf and 6 dpf in the livers of larsb^−/− larvae under Tg[fabp10:mcherry] background. Scale bar: 300 μm. (E) Quantification of liver size in larsb^−/− larvae under Tg[fabp10:mcherry] background (3 dpf and 6 dpf). Liver sizes were evaluated using ImageJ software version 1.52a (https://imagej.nih.gov/ij/). n = 5 fish/group. Error bars indicate SEM. Student’s t-test; ***P < 0.001. Statistics were calculated and the figure was produced in GraphPad software version 8 (https://www.graphpad.com/scientific-software/prism/). Larsb: leucyl-tRNA synthetase b, dpf: days post fertilization.

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Phenotype details

Acknowledgments

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