IMAGE

Figure 1

ID

ZDB-IMAGE-210424-2

Antibodies

Ab1-lars

Source

Figures for Inoue et al., 2021

Image

Figure Caption

Figure 1

Construction of larsb-knockout mutant zebrafish line. (A) Diagram showing the larsb genomic locus, CRISPR/Cas9 target site, and larsb-knockout (larsb^−/−) zebrafish mutant genotype. The sgRNA target sequence is displayed in green and the PAM region in red. In the genomic sequencing analysis chromatograms, the deletion region in the mutant larsb^−/− zebrafish is shown by the red box. (B) The Lars protein of larsb^−/− zebrafish had a missing editing domain. Western blot analysis of the Larsb protein expression in larsb^+/+ and larsb^−/− zebrafish. β-actin levels served as the loading control. Larsb: leucyl-tRNA synthetase b.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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