Fig. 10

Fig. 10 kzt phenotypes are rescued by overexpression of cygb1. Offspring embryos of matings between kzt heterozygotes were raised without plasmid injection (none, a–f), or injected with either pCAG (a’–f’, 20 pg/embryo) or pCAG-cygb1 (a’’–f’’, 20 pg/embryo). Heads of live embryos (live) or those stained with Alcian blue (AB) were observed at 4 dpf, and classified as normal (Normal), kzt-type mild (Mild), or kzt-type severe (Severe) phenotypes. Percentages of the respective observed phenotypes are shown at the bottom left, and the numbers of total embryos observed are shown on the right for each experimental group. Mild phenotypes were observed only when cygb1 was overexpressed. kzt-type phenotypes are indicated with arrows (heart), arrowheads (jaws), or two-headed white arrows (eyes). These data are included in Table 2 and Supplementary Fig. S11. bh, basihyal; cb, ceratobranchial; ch, ceratohyal; e, optic vesicle; hm, hyomandibular; mb, midbrain; mc, Meckel’s cartilage; ov, otic vesicle; pc, pericardial cavity. Scale bars, 200 ​μm.