Fig. 7

Fig. 7 Expression of cygb1 during zebrafish development. (A) The expression of cygb1 from the cleavage stage to 36 hpf in wild-type zebrafish embryos was examined by reverse transcription (RT)-PCR. The expected size of the product was 190 bp (arrowhead). ctrl, PCR product amplified from cygb1 cDNA plasmid. cygb1 expression increased from the bud stage to 15 hpf, plateauing thereafter. (B) cygb1 expression in embryos obtained from crosses of kzt heterozygotes was detected by WISH after the 3-somite stage (3-ss). (a, c, f, a’, c’, f’) Dorsal views with anterior to the left. (b, e, g, h, b’, e’, g’, h’) Lateral views with anterior to the left and dorsal to the top. (d, d’) Frontal views with dorsal to the top. At 3-ss and 18-ss, kzt homozygotes were difficult to identify morphologically, thus abnormal expression observed in a quarter of embryos was considered that of homozygotes. In these cases, the percentages of embryos with the expression shown and the numbers of total embryos examined are shown at the bottom. Cross-sectional views at the positions marked in (b/b’, c/c’, f/f’–h/h’) with dashed lines are shown below the corresponding panels. cygb1 expression (arrowheads) was extensively downregulated in kzt homozygotes at all stages examined. cnc, cranial neural crest cells; ccv, common cardinal vein; cv, cranial vessels; da, dorsal aorta; dlav, dorsal longitudinal anastomotic vessel; e, optic vesicle; fb, forebrain; h, heart; hb, hindbrain; mb, midbrain; ov, otic vesicle; pa, pharyngeal arch; pcv, posterior cardinal vein; phs, posterior head sinus; sm, somite; ∗, notochord. Scale bars, 200 ​μm.