|
Fig. 1 Diverse morphological defects are observed in kanazutsi (kzt) embryos. (a–f, a’–f’) Live images of kzt homozygotes (kzt–/–) and siblings (kzt+/) at 36 h post-fertilization (hpf), 48 hpf, and 4 days post-fertilization (dpf). kzt–/– embryos are characterized by bending of the body axis (arrowhead), swelling of the pericardial cavity (arrow), dwarf eyes (e, two-headed broken arrows), and flattening of the head (two-headed arrows). These phenotypes were more prominent at 4 dpf, and dysplasia of the upper and lower jaws was also observed (open arrowhead). Images are lateral views with anterior to the left and dorsal to the top. (g–j, g’–j’) Cartilage formation in kzt homozygotes (kzt–/–) and sibling embryos (kzt+/). Lateral views (g–j) and dorsal views (g’–j’) of embryos stained with Alcian blue show the defects in cranial cartilage development in kzt homozygotes. Regression of Meckel’s cartilage (mc) and ceratobranchial (cb) was remarkable, whereas ceratohyal (ch) was relatively normal. In addition, the angle between the left and right palatoquadrate cartilage (pq) in kzt mutants was obtuse compared to that in siblings. bh, basihyal; e, eye; et, ethmoid; h, heart; hb, hindbrain; hm, hyomandibular; mb, midbrain; oa, occipital arc; ov, otic vesicle; t, trabeculae; ta, tail, te, telencephalon. Scale bars, 200 μm.
Reprinted from Developmental Biology, 472, Takahashi, K., Ito, Y., Yoshimura, M., Nikaido, M., Yuikawa, T., Kawamura, A., Tsuda, S., Kage, D., Yamasu, K., A globin-family protein, cytoglobin 1, is involved in the development of neural crest-derived tissues and organs in zebrafish, 1-17, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.