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Figure 2—figure supplement 2. (A) Time-matched phalloidin stained WT, MZrab25a, and MZrab25b embryos at 1hpf; Yellow box shows disorganized cortical actin network. Scale bar 100 μm. (B,B’) EVL morphology does not resemble mutant embryos when WT yolk cell F-actin networks are perturbed. Stage-matched embryos positioned laterally at 6hpf stained with phalloidin. Scale bar 100 μm. (C) EVL defects emerge over the duration of epiboly in MZrab25b mutant embryos. Stage-matched WT and MZrab25b embryos positioned laterally stained with phalloidin. Scale bar 100 μm.

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