FIGURE 1

FIGURE 1

Generation of serpini1^−/− zebrafish via CRISPR-CAS9 technique. (A): Schematic representation of CRISPR/Cas9 target sites in zebrafish gene serpini1. The 19 bp target site is located on the exon4 of the serpini1 gene. (B): DNA sequencing results of the wild-type (WT) and homozygote serpini1^−/−. Serpini1^−/− mutation leads to five-nucleotide deletion in the fourth exon. (C): Protein comparison for WT and serpini1^−/− mutation. The serpini1^−/− caused a frameshift mutation, truncated from 415 aa into a 271 aa protein. (D). mRNA level of serpini1 in serpini1^−/− compared to WT. The data are presented as the mean ± SEM, N = 3 trails. Statistical analyses were performed with Student’s t-test, ****p < 0.0001 compared to WT.