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Fig. 6 Generation of a novel kif6 p.P293T mutant zebrafish. (A) Clustal W alignment of Human and Danio rerio Kif6 protein to highlight the P293T mutation (marked by asterisk). (B) Schematic of changes for donor editing of the kif6dp20 allele. (C) Sanger sequence of a stable heterozygous kif6dp20 mutant zebrafish. (D) Representative EcoR1 and allele specific genotyping of the kif6dp20 allele. (E, F) Representative phenotypes of a homozygous kif6dp20 mutant zebrafish at 30 dpf.
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Reprinted from Developmental Biology, 471, Gray, R.S., Gonzalez, R., Ackerman, S.D., Minowa, R., Griest, J.F., Bayrak, M.N., Troutwine, B., Canter, S., Monk, K.R., Sepich, D.S., Solnica-Krezel, L., Postembryonic screen for mutations affecting spine development in zebrafish, 18-33, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.