Schematics show organization of the Reissner fiber in respect of the ventricular system (based on Figs. 9, 10, and 11). a–c 48 hpf. a Controls. b Anti-Camel morpholino–mediated loss-of-function. c Camel mRNA–mediated gain-of-function. Green color, midline structures and some CVOs; red, RF and AFRU+ material. Abbreviations: afp, anterior floor plate; ap, area postrema; cc, central canal; cpIII, choroid plexus of the third ventricle; cpIV, choroid plexus of the fourth ventricle; d, diencephalon; e, epiphysis; erf, ectopic Reissner fiber; h, hindbrain; fo, flexural organ; fp, floor plate; m, midbrain; mrp, midbrain roof plate; opc, optocoele (Sylvius aqueduct); po, pineal organ; rf, Reissner fiber; sco, subcommissural organ; t, telencephalon; vIII, third ventricle; vIV, fourth ventricle
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