FIGURE 4

FIGURE 4

Depletion of Ccn6 expression in zebrafish skeletal muscle inhibits locomotion in response to stimulus. (A,B) Ccn6 depletion in skeletal muscle by ccn6 morpholino restricts the distance covered by zebrafish in response to stimulus as compared to the corresponding controls (control morpholino-injected and uninjected) at 48 and 72 h post-injection (n = 3). (C,D) Significantly less number of turns by ccn6 morpholino-injected fish as compared to the corresponding controls at 48 and 72 h post-injection (n = 3). Data are presented as the mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001; NS, not significant (Student’s t test). (E) Representation of the experimental setup.